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rabbit monoclonal antibodies against cdk2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal antibodies against cdk2
    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg <t>CDK2</t> in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
    Rabbit Monoclonal Antibodies Against Cdk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against cdk2/product/Cell Signaling Technology Inc
    Average 97 stars, based on 753 article reviews
    rabbit monoclonal antibodies against cdk2 - by Bioz Stars, 2026-02
    97/100 stars

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    1) Product Images from "E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster"

    Article Title: E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster

    Journal: Cells

    doi: 10.3390/cells13181535

    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
    Figure Legend Snippet: Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.

    Techniques Used: Flow Cytometry, Labeling, Two Tailed Test, Isolation, Western Blot, Standard Deviation, Expressing, Control



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    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg <t>CDK2</t> in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
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    rabbit monoclonal antibody against cdk2  (Cell Signaling Technology Inc)
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    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg <t>CDK2</t> in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
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    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg <t>CDK2</t> in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
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    Image Search Results


    Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.

    Journal: Cells

    Article Title: E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster

    doi: 10.3390/cells13181535

    Figure Lengend Snippet: Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.

    Article Snippet: The other antibodies used for Western blotting were monoclonal antibodies, including rabbit monoclonal antibodies against PCNA (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit monoclonal antibodies against Cyclin B1 (1:1000, ABclonal, Wuhan, China), rabbit monoclonal antibodies against CDK2 (1:1000, Cell Signaling Technology, Boston, MA, USA), mouse monoclonal antibodies against Histone H3 (1:5000, Proteintech, Chicago, IL, USA), and HRP-conjugated rabbit monoclonal against beta-Tubulin (1:10,000, Proteintech, Chicago, IL, USA).

    Techniques: Flow Cytometry, Labeling, Two Tailed Test, Isolation, Western Blot, Standard Deviation, Expressing, Control